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| Registros recuperados: 12 | |
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| Brandão,Paulo Eduardo; Scheffer,Karin; Villarreal,Laura Yaneth; Achkar,Samira; Oliveira,Rafael de Novaes; Fahl,Willian de Oliveira; Castilho,Juliana Galera; Kotait,Ivanete; Richtzenhain,Leonardo José. |
| This article reports on the identification of a group 2 coronavirus (BatCoV DR/2007) in a Desmodus rotundus vampire bat in Brazil. Phylogenetic analysis of ORF1b revealed that BatCoV DR/2007 originates from a unique lineage in the archetypical group 2 coronaviruses, as described for bat species elsewhere with putative importance in Public Health. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coronavirus; Vampire bat; RNA-dependent; RNA-polymerase. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000600003 |
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| Brandão,Paulo Eduardo. |
| Abstract This paper reports on an investigation of the role of codon usage evolution on the suggested bovine-to-human spillover of Bovine coronavirus (BCoV), an enteric/respiratory virus of cattle, resulting in the emergence of the exclusively respiratory Human coronavirus OC43 (HCoV-OC43). Analyses based on full genomes of BCoV and HCoV-OC43 and on both human and bovine mRNAs sequences of cholecystokinin (CCK) and surfactant protein 1 A (SFTP1-A), representing the enteric and respiratory tract codon usage, respectively, have shown natural selection leading to optimization or deoptimization of viral codon usage to the human enteric and respiratory tracts depending on the virus genes under consideration. A higher correlation was found for the nucleotide... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Codon usage; Coronavirus; Spillover; Coevolution. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400692 |
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| Fahl,Willian Oliveira; Carnieli Jr,Pedro; Castilho,Juliana Galera; Carrieri,Maria Luiza; Kotait,Ivanete; Iamamoto,Keila; Oliveira,Rafael Novaes; Brandão,Paulo Eduardo. |
| In Brazil, bats have been assigned an increasing importance in public health as they are important rabies reservoirs. Phylogenetic studies have shown that rabies virus (RABV) strains from frugivorous bats Artibeus spp. are closely associated to those from the vampire bat Desmodus rotundus, but little is known about the molecular diversity of RABV in Artibeus spp. The N and G genes of RABV isolated from Artibeus spp. and cattle infected by D. rotundus were sequenced, and phylogenetic trees were constructed. The N gene nucleotides tree showed three clusters: one for D. rotundus and two for Artibeus spp. Regarding putative N amino acid-trees, two clusters were formed, one for D. rotundus and another for Artibeus spp. RABV G gene phylogeny supported the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rabies Bat Artibeus spp.; Desmodus rotundus; Phylogeny; Molecular epidemiology. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600008 |
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| Macedo,Carla Isabel; Carnieli Jr,Pedro; Brandão,Paulo Eduardo; Rosa,Elizabeth S. Travassos da; Oliveira,Rafael de Novaes; Castilho,Juliana Galera; Medeiros,Rita; Machado,Rosangela Rocha; Oliveira,Rosely Cerqueira de; Carrieri,Maria Luiza; Kotait,Ivanete. |
| Rapid diagnosis of rabies in suspected human cases influences post-exposure prophylaxis for potential contacts of the patient and ensures appropriate patient management. Apart from the central nervous system (CNS), rabies virus (RABV) is usually present in small sensory nerves adjacent to hair follicles of infected humans. We used an RT-PCR, with primers targeted to the 3' terminal portion of the nucleoprotein gene (N), to test neck-skin samples of nine patients who had rabies in order to validate a diagnostic method that could serve as an additional tool for rabies diagnosis, particularly in antemortem samples. Six of eight postmortem samples were found to be positive for rabies by RT-PCR, and one of two samples collected antemortem was positive with this... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human rabies; Neck-skin; Diagnosis; RT-PCR; DNA sequencing. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000500008 |
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| Favaro,Patricia Filippsen; Fernandes,Wilson Roberto; Reischak,Dilmara; Brandão,Paulo Eduardo; Silva,Sheila Oliveira de Souza; Richtzenhain,Leonardo José. |
| Abstract Equine influenza is one of the major respiratory infectious diseases in horses. An equine influenza virus outbreak was identified in vaccinated and unvaccinated horses in a veterinary school hospital in São Paulo, SP, Brazil, in September 2015. The twelve equine influenza viruses isolated belonged to Florida Clade 1. The hemagglutinin and neuraminidase amino acid sequences were compared with the recent isolates from North and South America and the World Organisation for Animal Health recommended Florida Clade 1 vaccine strain. The hemagglutinin amino acid sequences had nine substitutions, compared with the vaccine strain. Two of them were in antigenic site A (A138S and G142R), one in antigenic site E (R62K) and another not in antigenic site... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equine influenza; H3N8; Hemagglutinin; Neuraminidase; Evolutionary analysis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200336 |
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| Jerez,José Antonio; Gregori,Fábio; Brandão,Paulo Eduardo; Rodriguez,Cesar Alejandro Rosales; Ito,Fumio Honma; Buzinaro,Maria da Glória; Sakai,Takeo. |
| Isolation of BCoV was performed in monolayers of HmLu-1 cells, using 20 fecal samples from clinical cases of diarrhea in calves. Samples were positive for BCoV by means of hemagglutination / hemagglutination inhibition (HA/HI). Up to the fifth serial passage, 13 of these isolates presented syncytial cytopathic effect, similar to Kakegawa standard strain. When isolates were submitted to seroneutralization with gammaglobulin anti-bovine coronavirus, 8 of them were considered to be positive, once cytopathic effect was neutralized. After titration and seroneutralization in microplates, only three of them were confirmed as positive. The lineage HmLu-1 showed higher permissivity to BCoV isolation, but the low intensity of viral replication demonstrated that new... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine coronavirus (BCoV); Isolation; HmLu-1 cells. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300001 |
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| Brandão,Paulo Eduardo; Cortez,Adriana; Ferreira,Fernando; Ferreira Neto,José Soares; Gregori,Fábio; Heinemann,Marcos Bryan; Homem,Valéria Stacchini Ferreira; Rodriguez,Cesar Alejandro Rosales; Jerez,José Antonio. |
| Rotavirus is a worldwide etiologic agent of diarrhea, responsible for large economic losses. We studied the seroprevalence of antibodies to group A rotavirus in cattle in 67 smallholder farms from Uruará municipality, using counterimmunoelectroosmophoresis with the NCDV strain as a standard antigen. Prevalence of positive smallholder farms was 95.6-100%. Significant differences were seen between age groups when the seropositivity rose from the youngest to the oldest groups and between females and males older than 1 year, when the seropositivity was higher in the first group. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seroepidemiologic studies; Antibodies; Rotavirus; Cattle; Amazonia. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962002000100003 |
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| Registros recuperados: 12 | |
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